In vitro degradation of dermal sheep collagen cross-linked using a water-soluble carbodiimide


Olde Damink, L.H.H. and Dijkstra, P.J. and Luyn, M.J.A. van and Wachem, P.B. van and Nieuwenhuis, P. and Feijen, J. (1996) In vitro degradation of dermal sheep collagen cross-linked using a water-soluble carbodiimide. Biomaterials, 17 (7). pp. 679-684. ISSN 0142-9612

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Abstract:Bacterial collagenase was used to study the susceptibility of dermal sheep collagen (DSC) crosslinked with a mixture of the water-soluble carbodiimide 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide hydrochloride and N-hydroxysuccinimide (E/N-DSC) towards enzymatic degradation. Contrary to non-cross-linked DSC (N-DSC), which had a rate of weight-loss of 18.1% per hour upon degradation, no weight loss was observed for E/N-DSC during a 24 h degradation period. The tensile strength of the E/N-DSC samples decreased during this time period, resulting in partially degraded samples having 80% of the initial tensile strength remaining. The susceptibility of E/N-DSC samples towards enzymatic degradation could be controlled by varying the degree of cross-linking of the samples. Ethylene oxide sterilization of E/N-DSC samples made the material more resistant against degradation compared with non-sterilized E/N-DSC samples. This may be explained by a decrease of the adsorption of bacterial collagenase onto the collagen owing to reaction of ethylene oxide with remaining free amine groups in the collagen matrix.
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Copyright:© 1996 Elsevier Science
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