The role of cellular fibronectin in the interaction of human endothelial cells with polymers


Dekker, A. and Beugeling, T. and Poot, A. and Spijkers, J. and Mourik, J.A. van and Feijen, J. and Bantjes, A. and Aken, W.G. van (1993) The role of cellular fibronectin in the interaction of human endothelial cells with polymers. Clinical Materials, 13 (1-4). pp. 101-107. ISSN 0267-6605

open access
Abstract:During in-vitro adhesion, spreading and proliferation of human endothelial cells (HEC) on tissue culture polystyrene (TCPS), cellular fibronectin is deposited onto the surface of TCPS in spite of the fact that relatively large amounts of proteins have been adsorbed from the serum-containing culture medium to this surface. Evidence is presented that serum proteins, adsorbed to the TCPS surface, are displaced by cellular fibronectin. In addition, the interaction of HEC with polyethylene, precoated with monoclonal antibodies directed against HEC membrane antigens and against extracellular matrix compounds, was studied. F(ab')2 fragments of two monoclonal antibodies were also included in this study. Preadsorption of these antibodies and F(ab')2 fragments resulted in cell adhesion and spreading as well as moderate cell proliferation (or no proliferation) for several days. A good cell proliferation of HEC was only observed on polyethylene precoated with fibronectin or an antibody directed against fibronectin. The results indicate that the direct or indirect deposition of fibronectin is a prerequisite for the proliferation of HEC. It is suggested that fibronectin, bound to a solid substrate, provides a biochemical signal necessary for the proliferation of HEC.
Item Type:Article
Copyright:© 1993 Elsevier Science
Science and Technology (TNW)
Research Group:
Link to this item:
Official URL:
Export this item as:BibTeX
HTML Citation
Reference Manager


Repository Staff Only: item control page

Metis ID: 105288