In vitro leucocyte adhesion to modified polyurethane surfaces. I. Effect of ionizable functional groups

Bruil, Anton and Terlingen, Johannes G.A. and Beugeling, Tom and Aken van, Willem G. and Feijen, Jan (1992) In vitro leucocyte adhesion to modified polyurethane surfaces. I. Effect of ionizable functional groups. Biomaterials, 13 (13). pp. 915-923. ISSN 0142-9612

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Abstract:	To study the effect of ionizable functional groups on the adhesion of leucocytes to surfaces, both poly(ethyleneimine) and poly(acrylic acid) were immobilized on polyurethane films, resulting in the introduction of amine and carboxylic acid groups, respectively. This was confirmed by contact angle measurements and XPS analysis. In vitro adhesion of granulocytes and lymphocytes on untreated and modified surfaces was compared. The number of adherent cells on modified surfaces as a function of time was significantly higher than on untreated surfaces. This effect was most pronounced for the adhesion of lymphocytes to surfaces modified with amine groups. In this case, the number of adherent cells after 1 h of exposure was three times higher than on untreated surfaces. A moderate enhancement of leucocyte adhesion was observed in the case of surfaces modified with carboxylic acid groups. There is evidence that these groups were not ionized under the experimental conditions used. The modification procedures described may be used to improve polyurethane filters for the removal of leucocytes from blood.
Item Type:	Article
Copyright:	© 1992 Elsevier Science
Faculty:	Science and Technology (TNW)
Research Group:	Polymer Chemistry and BioMaterials (PBM)
Link to this item:	http://purl.utwente.nl/publications/9631
Official URL:	http://dx.doi.org/10.1016/0142-9612(92)90114-4
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