Electrokinetic DNA transport in 20 nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel

Castillo-Fernandez, Oscar and Salieb-Beugelaar, Georgette B. and Nieuwkasteele van, Jan W. and Bomer, Johan G. and Arundell, Martin and Samitier, Josep and Berg van den, Albert and Eijkel, Jan C.T. (2012) Electrokinetic DNA transport in 20 nm-high nanoslits: Evidence for movement through a wall-adsorbed polymer nanogel. Electrophoresis, 32 (18). pp. 2404-2409. ISSN 0173-0835

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Abstract:	The electrokinetic transport behavior of lambda-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.
Item Type:	Article
Copyright:	© 2012 Wiley
Faculty:	Electrical Engineering, Mathematics and Computer Science (EEMCS)
Research Group:	Biomedical and Environmental Sensor systems (BIOS)
Link to this item:	http://purl.utwente.nl/publications/81913
Official URL:	http://dx.doi.org/10.1002/elps.201100278
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