Fluorescently Labeled Branched Polymers and Thermal Responsive Nanoparticles for Live Cell Imaging

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Zhou, D. and Ma, Y. and Poot, A.A. and Dijkstra, P.J. and Feijen, J. (2012) Fluorescently Labeled Branched Polymers and Thermal Responsive Nanoparticles for Live Cell Imaging. Macromolecular bioscience, 12 (4). 465 - 474. ISSN 1616-5187

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Abstract:Branched poly(methoxy-PEG acrylate) and thermally responsive poly(methoxy-PEG acrylate)-block-poly(N-isopropylacrylamide) are synthesized by RAFT polymerization. After reduction, these polymers are fluorescently labeled by reacting the free thiol groups with N-(5-fluoresceinyl)maleimide. As shown by DLS, the labeled copolymer poly(methoxy-PEG acrylate)-block-poly(N-isopropylacrylamide) forms nanoparticles at body temperature (37 °C) due to the presence of the thermosensitive poly(N-isopropylacrylamide). These materials were used as bioprobes for imaging HUVECs in vitro and chick embryo CAM in vivo. Both labeled polymer and nanoparticles are biocompatible and can be used as efficient fluorescent bioprobes
Item Type:Article
Copyright:© 2012 WILEY-VCH Verlag
Faculty:
Science and Technology (TNW)
Link to this item:http://purl.utwente.nl/publications/81775
Official URL:http://dx.doi.org/10.1002/mabi.201100254
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