Scattering gold nanoparticles: strategies for ultra sensitive DNA detection


Verdoold, R. (2012) Scattering gold nanoparticles: strategies for ultra sensitive DNA detection. thesis.

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Abstract:Dark field (DF) optical detection of gold nanoparticles (GNPs) is a single-molecule
method which can be used for sensitive and specific detection of nucleic acid
strands such as DNA and RNA. This thesis describes the development of a novel
biosensor based on scattering GNPs, from a theoretical idea on paper to a working
sensor system which is sensitive for specific DNA detection in the femtomolar range.
Furthermore, the modification chemistry and theoretical simulations are described
to provide insight into the reported sensing method and experimental setup.
The light scattering and absorption properties of GNPs can be utilised for the
detection of DNA. Binding of molecules to the GNP influences the local refractive
index. The increase in refractive index can be measured as a proportional red-shift
of the GNP extinction maximum; therefore GNPs are suitable for use as nanoparticle
chemical sensors. Utilizing this approach it is possible to detect DNA in naturally
occurring quantities. In total, five different DNA detection assays were investigated.
Item Type:Thesis
Science and Technology (TNW)
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