The noise-limited-resolution for stimulated emission depletion microscopy of diffusing particles

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Lee, C.J. and Boller, K.J. (2012) The noise-limited-resolution for stimulated emission depletion microscopy of diffusing particles. Virtual journal for biomedical optics, 7 (8). 1 - 6. ISSN 1931-1532

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Abstract:With recent developments in microscopy, such as stimulated emission depletion (STED) microscopy, far-field imaging at resolutions better than the diffraction limit is now a commercially available technique. Here, we show that, in the special case of a diffusive regime, the noise-limited resolution of STED imaging is independent of the saturation intensity of the fluorescent label. Thermal motion limits the signal integration time, which, for a given excited-state lifetime, limits the total number of photons available for detection.
Item Type:Article
Copyright:© 2012 OSA
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Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/81131
Official URL:http://dx.doi.org/10.1364/OE.20.012793
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Metis ID: 287513