In vitro and in vivo evaluation of the inflammatory response to nanoscale grooved substrates

Share/Save/Bookmark

Lamers, E. and Walboomers, X.F. and Domanski, M. and Prodanov, L. and Melis, J. and Luttge, R. and Winnubst, A.J.A. and Anderson, J.M. and Gardeniers, J.G.E. and Jansen, J.A. (2012) In vitro and in vivo evaluation of the inflammatory response to nanoscale grooved substrates. Nanomedicine : nanotechnology, biology and medicine, 8 (3). 308 - 317. ISSN 1748-6963

[img] PDF
Restricted to UT campus only
: Request a copy
1MB
Abstract:The immune response to an implanted biomaterial is orchestrated by macrophages. In this study various nanogrooved patterns were created by using laser interference lithography and reactive ion etching. The created nanogrooves mimic the natural extracellular matrix environment. Macrophage cell culture demonstrated that interleukin 1β and TNF-α cytokine production were upregulated on nanogrooved substrates. In vivo subcutaneous implantation in a validated mouse cage model for 14 days demonstrated that nanogrooves enhanced and guided cell adhesion, and few multinucleated cells were formed. In agreement with the in vitro results, cytokine production was found to be nanogroove dependent, as interleukin 1β, TNF-α, TGF-β and osteopontin became upregulated. The results indicate that biomaterial surface texturing, especially at the nanometric scale, can be used to control macrophage activation to induce a wound healing response, rather than a profound inflammatory response.
Item Type:Article
Copyright:© Elsevier
Faculty:
Science and Technology (TNW)
Research Group:
Link to this item:http://purl.utwente.nl/publications/81068
Official URL:http://dx.doi.org/10.1016/j.nano.2011.06.013
Export this item as:BibTeX
EndNote
HTML Citation
Reference Manager

 

Repository Staff Only: item control page

Metis ID: 286754