Non-invasive imaging of hypoxia in tissue engineering


Liu, Jun and Blitterswijk, Clemens van and Martens, Anton and Boer, Jan de (2007) Non-invasive imaging of hypoxia in tissue engineering. European Cells & Materials, 14 (Suppl. 1). p. 8. ISSN 1473-2262

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Abstract:In tissue engineering, cells are grown on biomaterials in vitro and subsequently implanted. A critical parameter in effective proliferation and differentiation is the availability of nutrients. Few tools are currently available to monitor the nutritional status of cells. In this study, we have employed A4-4 cells [1], a Chinese hamster ovary cell line stably transfected with a luciferase gene driven by the hypoxia responsive element (HRE) from the promoter region of the VEGF gene [2, 3]. HRE activity, and thus luciferase activity, directly correlates with decreasing cellular O2 levels.The aim of this study is to investigate whether the HREluciferase construct can be used for non-invasive imaging of hypoxia in tissue engineering.
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Additional information:Abstract of European Cells and Materials VIII Bone Tissue Engineering June 25th - 28th 2007, Congress Centre, Davos, Switzerland
Science and Technology (TNW)
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