Effects of soluble cobalt and cobalt incorporated into calcium phosphate layers on osteoclast differentiation and activation

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Patntirapong, Somying and Habibovic, Pamela and Hauschka, Peter V. (2009) Effects of soluble cobalt and cobalt incorporated into calcium phosphate layers on osteoclast differentiation and activation. Biomaterials, 30 (4). pp. 548-555. ISSN 0142-9612

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Abstract:Metal ions originating from mechanical debris and corrosive wear of prosthetic implant alloys accumulate in peri-implant soft tissues, bone mineral, and body fluids. Eventually, metal ions such as cobalt (II) (Co2+), which is a major component of cobalt–chromium-based implant alloys and a known activator of osteolysis, are incorporated into the mineral phase of bone. We hypothesize that the accumulation of Co2+ in the mineral could directly activate osteolysis by targeting osteoclasts. To test this hypothesis, we coated tissue culture plastic with a thin layer of calcium phosphate (CaP) containing added traces of Co2+, thereby mimicking the bone mineral accumulation of Co2+. Murine bone marrow osteoclasts formed in the presence of M-CSF and RANKL were cultured on these surfaces to examine the effects of Co2+ on osteoclast formation and resorptive activity. Treatment conditions with Co2+ involved incorporation into the CaP layer, adsorption to the mineral surface, or addition to culture media. Micromolar concentrations of Co2+ delivered to developing osteoclast precursors by all 3 routes increased both osteoclast differentiation and resorptive function. Compared to CaP layers without Co2+, we observed a maximal 75% increase in osteoclast numbers and a 2.3- to 2.7-fold increase in mineral resorption from the tissue culture wells containing 0.1 μm Co2+ and 0.1–10 μm Co2+, respectively. These concentrations are well within the range found in peri-implant tissues in vivo. This direct effect of Co2+ on osteoclasts appears to act independently of the particulate phagocytosis/inflammation-mediated pathways, thus enhancing osteolysis and aseptic implant loosening.
Item Type:Article
Copyright:© 2009 Elsevier
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Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/80152
Official URL:http://dx.doi.org/10.1016/j.biomaterials.2008.09.062
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