Host–guest extraction of immunoglobulin G using calix[6]arenas

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Martínez-Aragón, M. and Goetheer, E.L.V. and Haan, A.B. de (2009) Host–guest extraction of immunoglobulin G using calix[6]arenas. Separation and Purification Technology, 65 (1). pp. 73-78. ISSN 1383-5866

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Abstract:Host–guest interactions have been widely employed in various techniques in biochemistry in the last decades. In this way, the use of calix[6]arenes modified with a carboxylic acid as host in the host–guest extraction of immunoglobulin G (IgG) is studied. It is possible to extract IgG from an aqueous medium. Different operational parameters such as pH, salt concentration and stirring speed were evaluated. At high pH, up to 50% of the IgG can be transferred to the organic phase. The study of pH demonstrates that the interaction mechanism depends on a proton exchange mechanism together with hydrogen-bonding. Salt concentration has a direct effect on the interaction strength of the lysine residues of IgG and the calix[6]arene, leading to a diminished extraction at high salt concentrations. For the prevention of denaturation the contact mode and intensity of mixing are crucial. Based on the data obtained fromkinetic and equilibrium studies a suggestion for the extraction mechanism is made.
Item Type:Article
Copyright:© 2009 Elsevier
Faculty:
Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/80095
Official URL:http://dx.doi.org/10.1016/j.seppur.2008.03.017
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