Host–guest extraction of immunoglobulin G using calix[6]arenas


Martínez-Aragón, M. and Goetheer, E.L.V. and Haan, A.B. de (2009) Host–guest extraction of immunoglobulin G using calix[6]arenas. Separation and Purification Technology, 65 (1). pp. 73-78. ISSN 1383-5866

[img] PDF
Restricted to UT campus only
: Request a copy
Abstract:Host–guest interactions have been widely employed in various techniques in biochemistry in the last decades. In this way, the use of calix[6]arenes modified with a carboxylic acid as host in the host–guest extraction of immunoglobulin G (IgG) is studied. It is possible to extract IgG from an aqueous medium. Different operational parameters such as pH, salt concentration and stirring speed were evaluated. At high pH, up to 50% of the IgG can be transferred to the organic phase. The study of pH demonstrates that the interaction mechanism depends on a proton exchange mechanism together with hydrogen-bonding. Salt concentration has a direct effect on the interaction strength of the lysine residues of IgG and the calix[6]arene, leading to a diminished extraction at high salt concentrations. For the prevention of denaturation the contact mode and intensity of mixing are crucial. Based on the data obtained fromkinetic and equilibrium studies a suggestion for the extraction mechanism is made.
Item Type:Article
Copyright:© 2009 Elsevier
Science and Technology (TNW)
Research Group:
Link to this item:
Official URL:
Export this item as:BibTeX
HTML Citation
Reference Manager


Repository Staff Only: item control page