High fidelity neuronal networks formed by plasma masking with a bilayer membrane: analysis of neurodegenerative and neuroprotective processes

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Hardelauf, Heike and Sisnaiske, Julia and Taghipour-Anvari, Amir Ali and Jacob, Peter and Drabiniok, Evelyn and Marggraf, Ulrich and Frimat, Jean-Philippe and Hengstler, Jan G. and Neyer, Andreas and Thriel, Christoph van and West, Jonathan (2011) High fidelity neuronal networks formed by plasma masking with a bilayer membrane: analysis of neurodegenerative and neuroprotective processes. Lab on a Chip, 11 (16). pp. 2763-2771. ISSN 1473-0197

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Abstract:Spatially defined neuronal networks have great potential to be used in a wide spectrum of neurobiology assays. We present an original technique for the precise and reproducible formation of neuronal networks. A PDMS membrane comprising through-holes aligned with interconnecting microchannels was used during oxygen plasma etching to dry mask a protein rejecting poly(ethylene glycol) (PEG) adlayer. Patterns were faithfully replicated to produce an oxidized interconnected array pattern which supported protein adsorption. Differentiated human SH-SY5Y neuron-like cells adhered to the array nodes with the micron-scale interconnecting tracks guiding neurite outgrowth to produce neuronal connections and establish a network. A 2.0 μm track width was optimal for high-level network formation and node compliance. These spatially standardized neuronal networks were used to analyse the dynamics of acrylamide-induced neurite degeneration and the protective effects of co-treatment with calpeptin or brain derived neurotrophic factor (BDNF).
Item Type:Article
Copyright:© 2011 RSC Publishing
Faculty:
Electrical Engineering, Mathematics and Computer Science (EEMCS)
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Link to this item:http://purl.utwente.nl/publications/78993
Official URL:http://dx.doi.org/10.1039/c1lc20257j
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