Microfluidic DNA fragmentation for on-chip genomic analysis

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Shui, Lingling and Bomer, Johan G. and Jin, Mingliang and Carlen, Edwin T. and Berg, Albert van den (2011) Microfluidic DNA fragmentation for on-chip genomic analysis. Nanotechnology, 22 (49). pp. 494013-494019. ISSN 0957-4484

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Abstract:We report a high-throughput clog-free microfluidic deoxyribonucleic acid (DNA) fragmentation chip that is based on hydrodynamic shearing. Salmon sperm DNA has been reproducibly fragmented down to ∼5k bp fragment lengths by applying low hydraulic pressures (≤1 bar) across micromachined constrictions positioned in larger microfluidic channels that create point-sink flow with large velocity gradients near the constriction entrance. Long constrictions (100 μm) produce shorter fragment lengths compared to shorter constrictions (10 μm), while increasing the hydrodynamic pressure requirement. Sample recirculation (10×) in short constrictions reduces the mean fragment length and fragment length variation, and improves yield compared to single-pass experiments without increasing the hydrodynamic pressure.
Item Type:Article
Copyright:© 2011 IOP Publishing
Faculty:
Electrical Engineering, Mathematics and Computer Science (EEMCS)
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Link to this item:http://purl.utwente.nl/publications/78738
Official URL:http://dx.doi.org/10.1088/0957-4484/22/49/494013
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