Low pressure microfluidic-based DNA fragmentation


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Shui, Lingling and Sparreboom, Wouter and Bomer, Johan G. and Jin, Mingliang and Carlen, Edwin T. and Berg van den, Albert (2011) Low pressure microfluidic-based DNA fragmentation. In: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011, 2-6 October 2011, Seattle, Washington.

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Abstract:We report a low-pressure microfluidic deoxyribonucleic acid (DNA) fragmentation device based on a combination of me-chanical hydrodynamic shearing and low temperature sample heating. Conventional DNA fragmentation based on hydrody-namic shearing is capable of achieving fragment lengths (FL) < 10k bp with pressures ΔP > 1 bar. The application of a small amount of additional thermal energy to the buffer medium results in ~10x reduction in pressure requirement resulting in FL~6k-8k bp with ΔP~1-0.1 bar with sample temperature Ts~45º C
Item Type:Conference or Workshop Item
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Electrical Engineering, Mathematics and Computer Science (EEMCS)
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Link to this item:http://purl.utwente.nl/publications/78659
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