Low pressure microfluidic-based DNA fragmentation


Shui, Lingling and Sparreboom, Wouter and Bomer, Johan G. and Jin, Mingliang and Carlen, Edwin T. and Berg, Albert van den (2011) Low pressure microfluidic-based DNA fragmentation. In: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2011, 2-6 October 2011, Seattle, Washington (pp. pp. 1191-1193).

[img] PDF
Restricted to UT campus only
: Request a copy
Abstract:We report a low-pressure microfluidic deoxyribonucleic acid (DNA) fragmentation device based on a combination of me-chanical hydrodynamic shearing and low temperature sample heating. Conventional DNA fragmentation based on hydrody-namic shearing is capable of achieving fragment lengths (FL) < 10k bp with pressures ΔP > 1 bar. The application of a small amount of additional thermal energy to the buffer medium results in ~10x reduction in pressure requirement resulting in FL~6k-8k bp with ΔP~1-0.1 bar with sample temperature Ts~45º C
Item Type:Conference or Workshop Item
Electrical Engineering, Mathematics and Computer Science (EEMCS)
Research Group:
Link to this item:http://purl.utwente.nl/publications/78659
Export this item as:BibTeX
HTML Citation
Reference Manager


Repository Staff Only: item control page