Biocatalytic oxidation by chloroperoxidase from Caldariomyces fumago in polymersome nanoreactors

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Hoog de, H.M. and Nallani, M. and Cornelissen, J.J.L.M. and Rowan, A.E. and Nolte, J.M. and Arends, I.W.C.E. (2009) Biocatalytic oxidation by chloroperoxidase from Caldariomyces fumago in polymersome nanoreactors. Organic and Biomolecular Chemistry, 7 . pp. 4604-4610. ISSN 1477-0520

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Abstract:The encapsulation of chloroperoxidase from Caldariomyces fumago (CPO) in block copolymer polymersomes is reported. Fluorescence and electron microscopy show that when the encapsulating conditions favour self-assembly of the block copolymer, the enzyme is incorporated with concentrations that are 50 times higher than the enzyme concentration before encapsulation. The oxidation of two substrates by the encapsulated enzyme was studied: i) pyrogallol, a common substrate used to assay CPO enzymatic activity and ii) thioanisole, of which the product, (R)-methyl phenyl sulfoxide, is an important pharmaceutical intermediate. The CPO-loaded polymersomes showed distinct reactivity towards these substrates. While the oxidation of pyrogallol was limited by diffusion of the substrate into the polymersome, the rate-limiting step for the oxidation of thioansiole was the turnover by the enzyme
Item Type:Article
Copyright:© Royal Society of Chemistry 2011
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Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/77781
Official URL:http://dx.doi.org/10.1039/B911370C
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