Vibrational phase contrast CARS microscopy for quantitative analysis

Jurna, M. and Garbacik, E.T. and Korterik, J.P. and Otto, C. and Herek, J.L. and Offerhaus, H.L. (2010) Vibrational phase contrast CARS microscopy for quantitative analysis. In: Multiphoton Microscopy in the Biomedical Sciences X. Proceedings of SPIE, 7569 . SPIE--The International Society for Optical Engineering, 75690F. ISBN 9780819479655

PDF
Restricted to UT campus only
1055Kb

Abstract:	In biological samples the resonant CARS signal of less abundant constituents can be overwhelmed by the nonresonant background, preventing detection of those molecules. We demonstrate a method to obtain the phase of the oscillators in the focal volume that allows discrimination of those hidden molecules. The phase is measured with respect to the local excitation fields using a cascaded phase-preserving chain. It is measured point-bypoint and takes into account refractive index changes in the sample, phase curvature over the field-of-view and interferometric instabilities. The detection of the phase of the vibrational motion can be regarded as a vibrational extension of the linear (refractive index) phase contrast microscopy introduced by Zernike around 1933
Item Type:	Book Section
Copyright:	© 2010 COPYRIGHT SPIE--The International Society for Optical Engineering
Faculty:	Science and Technology (TNW)
Research Group:	Optical Sciences (OS)
Link to this item:	http://purl.utwente.nl/publications/77699
Official URL:	http://dx.doi.org/10.1117/12.840178
Export this item as:	BibTeX EndNote HTML Citation Reference Manager

Show download statistics for this publication

Repository Staff Only: item control page

Metis ID: 267055