Quantitative measurement of attenuation coefficients of bladder biopsies using optical coherence tomography for grading urothelial carcinoma of the bladder

Cauberg, Evelyne C.C. and Reijke de, Theo M. and Rosette de la, Jean J.M.C.H. and Bruin de, Daniel M. and Faber, Dirk J. and Visser, Mike and Leeuwen van, Ton G. (2010) Quantitative measurement of attenuation coefficients of bladder biopsies using optical coherence tomography for grading urothelial carcinoma of the bladder. Journal of Biomedical Optics, 15 (066013). 066013. ISSN 1083-3668

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Abstract:	Real-time grading of bladder urothelial carcinoma (UC) is clinically important, but the current standard for grading (histopathology) cannot provide this information. Based on optical coherence tomography (OCT)-measured optical attenuation (μt), the grade of bladder UC could potentially be assessed in real time. We evaluate ex vivo whether μt differs between different grades of UC and benign bladder tissue. Human bladder tissue specimens are examined ex vivo by 850-nm OCT using dynamic focusing. Three observers independently determine the μt from the OCT images, and three pathologists independently review the corresponding histology slides. For both methods, a consensus diagnosis is made. We include 76 OCT scans from 54 bladder samples obtained in 20 procedures on 18 patients. The median (interquartile range) μt of benign tissue is 5.75 mm−1 (4.77 to 6.14) versus 5.52 mm−1 (3.47 to 5.90), 4.85 mm−1 (4.25 to 6.50), and 5.62 mm−1 (5.01 to 6.29) for grade 1, 2, and 3 UC, respectively (p = 0.732). Interobserver agreement of histopathology is “substantial” [Kappa 0.62, 95% confidence interval (IC) 0.54 to 0.70] compared to “almost perfect” [interclass correlation coefficient (ICC) 0.87, 95% CI 0.80 to 0.92] for OCT. Quantitative OCT analysis (by μt) does not detect morphological UC changes. This may be due to factors typical for an ex-vivo experimental setting
Item Type:	Article
Copyright:	© 2010 Society of Photo-Optical Instrumentation Engineers (SPIE)
Faculty:	Science and Technology (TNW)
Research Group:	Biomedical Photonic Imaging (BMPI)
Link to this item:	http://purl.utwente.nl/publications/77693
Official URL:	http://dx.doi.org/10.1117/1.3512206
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