Dual bio-responsive gene delivery via reducible poly(amido amine) and survivin-inducible plasmid DNA

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Namgung, Ran and Brumbach, Jonathan H. and Jeong, Ji Hoon and Yockman, James W. and Kim, Sung Wan and Lin, Chao and Zhong, Zhiyuan and Feijen, Jan and Engbersen, Johan F.J. and Kim , Won Jong (2010) Dual bio-responsive gene delivery via reducible poly(amido amine) and survivin-inducible plasmid DNA. Biotechnology letters, 32 (6). pp. 755-764. ISSN 0141-5492

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Abstract:A bioreducible poly(amido amine) (SS-PAA) gene carrier, known as poly (amido-butanol) (pABOL), was used to transfect a variety of cancer and non-cancer cell lines. To obtain cancer-specific transgene expression for therapeutic efficiency in cancer treatment, we constructed survivin-inducible plasmid DNA expressing the soluble VEGF receptor, sFlt-1, downstream of the survivin promoter (pSUR-sFlt-1). Cancer-specific expression of sFlt-1 was observed in the mouse renal carcinoma (RENCA) cell line. pABOL enhanced the efficiency of gene delivery compared to traditional carriers used in the past. Thus, a dual bio-responsive gene delivery system was developed by using bioreducible p(ABOL) for enhanced intracellular gene delivery and survivin-inducible gene expression system (pSUR-sFlt-1 or pSUR-Luc reporter gene) that demonstrates increased gene expression in cancer that has advantages over current gene delivery systems
Item Type:Article
Copyright:© Springer
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Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/77536
Official URL:http://dx.doi.org/10.1007/s10529-010-0219-7
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