The influence of the scaffold design on the distribution of adhering cells after perfusion cell seeding

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Melchels, Ferry P.W. and Tonnarelli, Beatrice and Olivares, Andy L. and Martin, Ivan and Lacroix, Damien and Feijen, Jan and Wendt, David J. and Grijpma, Dirk W. (2011) The influence of the scaffold design on the distribution of adhering cells after perfusion cell seeding. Biomaterials, 32 (11). pp. 2878-2884. ISSN 0142-9612

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Abstract:In natural tissues, the extracellular matrix composition, cell density and physiological properties are often non-homogeneous. Here we describe a model system, in which the distribution of cells throughout tissue engineering scaffolds after perfusion seeding can be influenced by the pore architecture of the scaffold. Two scaffold types, both with gyroid pore architectures, were designed and built by stereolithography: one with isotropic pore size (412 ± 13 μm) and porosity (62 ± 1%), and another with a gradient in pore size (250–500 μm) and porosity (35%–85%). Computational fluid flow modelling showed a uniform distribution of flow velocities and wall shear rates (15–24 s-1) for the isotropic architecture, and a gradient in the distribution of flow velocities and wall shear rates (12–38 s-1) for the other architecture. The distribution of cells throughout perfusion-seeded scaffolds was visualised by confocal microscopy. The highest densities of cells correlated with regions of the scaffolds where the pores were larger, and the fluid velocities and wall shear rates were the highest. Under the applied perfusion conditions, cell deposition is mainly determined by local wall shear stress, which, in turn, is strongly influenced by the architecture of the pore network of the scaffold.

Item Type:Article
Copyright:© 2011 Elsevier
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Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/77516
Official URL:http://dx.doi.org/10.1016/j.biomaterials.2011.01.023
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