Oriented Immobilization of Farnesylated Proteins by the Thiol-Ene Reaction

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Weinrich, Dirk and Lin, Po-Chiao and Jonkheijm, Pascal and Nguyen, Uyen T.T. and Schröder, Hendrik and Niemeyer, Christof M. and Alexandrov, Kirill and Goody, Roger and Waldmann, Herbert (2010) Oriented Immobilization of Farnesylated Proteins by the Thiol-Ene Reaction. Angewandte Chemie, International Edition in English, 49 (7). pp. 1252-1257. ISSN 0570-0833

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Abstract:Anchoring the protein: Proteins were immobilized rapidly under mild conditions by thiol-ene photocoupling between S-farnesyl groups attached to a genetically encodable “CAAX-box” tetrapeptide sequence (A is aliphatic) at the C terminus of the protein and surface-exposed thiols (see scheme). This method enables the oriented covalent immobilization of proteins directly from expression lysates without additional purification or derivatization steps.
Item Type:Article
Copyright:© 2010 Wiley
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Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/76904
Official URL:http://dx.doi.org/10.1002/anie.200906190
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Metis ID: 273437