Oriented Immobilization of Farnesylated Proteins by the Thiol-Ene Reaction


Weinrich, Dirk and Lin, Po-Chiao and Jonkheijm, Pascal and Nguyen, Uyen T.T. and Schröder, Hendrik and Niemeyer, Christof M. and Alexandrov, Kirill and Goody, Roger and Waldmann, Herbert (2010) Oriented Immobilization of Farnesylated Proteins by the Thiol-Ene Reaction. Angewandte Chemie, International Edition in English, 49 (7). pp. 1252-1257. ISSN 0570-0833

[img] PDF
Restricted to UT campus only
: Request a copy
Abstract:Anchoring the protein: Proteins were immobilized rapidly under mild conditions by thiol-ene photocoupling between S-farnesyl groups attached to a genetically encodable “CAAX-box” tetrapeptide sequence (A is aliphatic) at the C terminus of the protein and surface-exposed thiols (see scheme). This method enables the oriented covalent immobilization of proteins directly from expression lysates without additional purification or derivatization steps.
Item Type:Article
Copyright:© 2010 Wiley
Science and Technology (TNW)
Research Group:
Link to this item:http://purl.utwente.nl/publications/76904
Official URL:https://doi.org/10.1002/anie.200906190
Export this item as:BibTeX
HTML Citation
Reference Manager


Repository Staff Only: item control page

Metis ID: 273437