Patterning of Peptide Nucleic Acids Using Reactive Microcontact Printing

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Calabretta, Alessandro and Wasserberg, Dorothee and Posthuma-Trumpie, Geertruida A. and Subramaniam, Vinod and Amerongen, Aart van and Corradini, Roberto and Tedeschi, Tullia and Sforza, Stefano and Reinhoudt, David N. and Marchelli, Rosangela and Huskens, Jurriaan and Jonkheijm, Pascal (2010) Patterning of Peptide Nucleic Acids Using Reactive Microcontact Printing. Langmuir, 27 (11). pp. 1536-1542. ISSN 0743-7463

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Abstract:PNAs (peptide nucleic acids) have been immobilized onto surfaces in a fast, accurate way by employing reactive microcontact printing. Surfaces have been first modified with aldehyde groups to react with the amino end of the synthesized PNAs. When patterning fluorescein-labeled PNAs by reactive microcontact printing using oxygen-oxidized polydimethylsiloxane stamps, homogeneous arrays were fabricated and characterized using optical methods. PNA-patterned surfaces were hybridized with complementary and mismatched dye-labeled oligonucleotides to test their ability to recognize DNA sequences. The stability and selectivity of the PNA-DNA duplexes on surfaces have been verified by fluorescence microscopy, and the melting curves have been recorded. Finally, the technique has been applied to the fabrication of chips by spotting a PNA microarray onto a flat PDMS stamp and reproducing the same features onto many slides. The chips were finally applied to single nucleotide polymorphism detection on oligonucleotides.
Item Type:Article
Copyright:© 2010 American Chemical Society
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Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/76903
Official URL:http://dx.doi.org/10.1021/la102756k
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