Determination of aldehydes and ketones using derivatization with 2,4-dinitrophenylhydrazine and liquid chromatography–atmospheric pressure photoionization-mass spectrometry

Leeuwen van, Suze M. and Hendriksen, Laurens and Karst, Uwe (2004) Determination of aldehydes and ketones using derivatization with 2,4-dinitrophenylhydrazine and liquid chromatography–atmospheric pressure photoionization-mass spectrometry. Journal of Chromatography A, 1058 (1-2). pp. 107-112. ISSN 0021-9673

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Abstract:	Atmospheric pressure photoionization-mass spectrometry (APPI-MS) is used for the analysis of aldehydes and ketones after derivatization with 2,4-dinitrophenylhydrazine (DNPH) and liquid chromatographic separation. In the negative ion mode, the [M − H]− pseudomolecular ions are most abundant for the carbonyls. Compared with the established atmospheric pressure chemical ionization (APCI)-MS, limits of detection are typically lower using similar conditions. Automobile exhaust and cigarette exhaust samples were analyzed with APPI-MS and APCI-MS in combination with an ion trap mass analyzer. Due to improved limits of detection, more of the less abundant long-chain carbonyls are detected with APPI-MS in real samples. While 2,4-dinitrophenylazide, a known reaction product of DNPH with nitrogen dioxide, is detected in APCI-MS due to dissociative electron capture, it is not observed at all in APPI-MS.
Item Type:	Article
Copyright:	© 2004 Elsevier
Faculty:	Science and Technology (TNW)
Research Group:	Chemical Analysis (CA)
Link to this item:	http://purl.utwente.nl/publications/76287
Official URL:	http://dx.doi.org/10.1016/j.chroma.2004.08.149
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