High-throughput on-chip DNA fragmentation


Shui, L. and Jin, M. and Bomer, J.G. and Carlen, E.T. and Berg, A. van den (2010) High-throughput on-chip DNA fragmentation. In: 21st Micromechanics and Micro Systems Europe Workshop, MME 2010, September 26-29, 2010, Enschede, The Netherlands (pp. pp. 124-127).

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Abstract:free microfluidic deoxyribonucleic acid (DNA) fragmentation chip that is based on hydrodynamic shearing. Genomic DNA has been reproducibly fragmented with 2-10 kbp fragment lengths by applying hydraulic pressure ΔP across micromachined constrictions in the microfluidic channels. The utilization of a series of constrictions reduces the variance of the fragmented DNA length distribution; and parallel microfluidic channels design eliminates the device clogging.
Item Type:Conference or Workshop Item
Electrical Engineering, Mathematics and Computer Science (EEMCS)
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