Microfluidic systems for improving assisted reproductive technologies culture protocols


Rossem, F. van and Esteves, T.C. and Boiani, M. and Le Gac, S. and Berg, A. van den (2010) Microfluidic systems for improving assisted reproductive technologies culture protocols. In: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010, 3-7 October 2010, Groningen, the Netherlands (pp. pp. 1484-1486).

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Abstract:Despite extensive and increasing use, Assisted Reproduction Technologies exhibit low successful full-term pregnancy rate (< 30%). One weakness identified is the need of in vitro culturing embryos during pre-implantation stages, before embryos are transferred. Here, we propose a novel microfluidic system to address the issues surrounding culture effects on embryo development. We demonstrate that mouse embryos cultured in microchambers (30, 270 nL) develop faster and to higher blastocyst rates than in conventional systems (Nunc dishes, 400 μL). Also, single embryo culture in microfluidic systems is feasible. Finally, on-chip culture embryos develop viably (pups) after implantation in pseudopregnant mice.
Item Type:Conference or Workshop Item
Electrical Engineering, Mathematics and Computer Science (EEMCS)
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Link to this item:http://purl.utwente.nl/publications/75866
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