BLM Experimentation and opto-electrical characterization in microchips, towards an integrated platform for drug screening on membrane proteins


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Stimberg, V.C. and Uitert van, I. and Le Gac, S. and Berg van den, A. (2010) BLM Experimentation and opto-electrical characterization in microchips, towards an integrated platform for drug screening on membrane proteins. In: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2010, 3-7 October 2010, Groningen, the Netherlands.

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Abstract:Experimentation on cell membranes and on membrane proteins commonly makes use of planar and simplified membrane models, or bilayer lipid membranes (BLMs). Although these models are extensively employed, the experimentation is tedious and time-consuming, and mostly limited to electrical measurements. We propose here a dedicated microfluidic platform for BLM experimentation using electrical and optical techniques. BLMs are formed in the closed microdevice by exposing a lipid plug to air and buffer to give a bilayer structure. BLMs are highly stable, reproducible and have an excellent sealing resistance. Single protein studies are shown using α-hemolysin as a protein model.
Item Type:Conference or Workshop Item
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Electrical Engineering, Mathematics and Computer Science (EEMCS)
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Link to this item:http://purl.utwente.nl/publications/75864
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