Cell Capture and Lysis on a Chip


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Le Gac, Séverine and Berg, Albert van den (2010) Cell Capture and Lysis on a Chip. In: Unravelling Single Cell Genomics: Micro and Nanotools. RSC Nanoscience & Nanotechnology, 15 . Royal Society of Chemistry, Cambridge, pp. 150-184. ISBN 9781847559111

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Abstract:Single cell analysis in a microfluidic device proceeds through a certain number of steps. The two first steps of the protocol are the manipulation of individual
cells in the microfluidic device and their positioning in precise locations and subsequently the process to rupture the cell membrane to retrieve the cellular content. In this chapter, we will focus on these two first steps of cell trapping and single cell lysis, and review different approaches and techniques found in the literature for these two steps to achieve these in a microfluidic format. We will also discuss the advantages and limitations of the different techniques of manipulation, trapping and lysis.
Item Type:Book Section
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Electrical Engineering, Mathematics and Computer Science (EEMCS)
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Link to this item:http://purl.utwente.nl/publications/75854
Official URL:http://dx.doi.org/10.1039/9781849732284
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