Modulation-frequency encoded multi-color fluorescent DNA analysis in an optofluidic chip


Dongre, Chaitanya and Weerd, Jasper van and Besselink, Geert A.J. and Martinez Vazquez, Rebecca and Osellame, Roberto and Cerullo, Giulio and Weeghel, Rob van and Vlekkert, Hans H. van den and Hoekstra, Hugo J.W.M. and Pollnau, Markus (2010) Modulation-frequency encoded multi-color fluorescent DNA analysis in an optofluidic chip. Lab on a Chip, 11 (4). pp. 679-683. ISSN 1473-0197

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Abstract:We introduce a principle of parallel optical processing to an optofluidic lab-on-a-chip. During electrophoretic separation, the ultra-low limit of detection achieved with our set-up allows us to record fluorescence from covalently end-labeled DNA molecules. Different sets of exclusively color-labeled DNA fragments—otherwise rendered indistinguishable by spatio-temporal coincidence—are traced back to their origin by modulation-frequency-encoded multi- avelength laser excitation, fluorescence detection with a single ultrasensitive, albeit color-blind photomultiplier, and Fourier analysis decoding. As a proof of principle, fragments obtained by multiplex ligation-dependent probe amplification from independent human genomic segments, associated with genetic predispositions to breast cancer and anemia, are simultaneously analyzed.
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Copyright:© 2010 The Royal Society of Chemistry
Electrical Engineering, Mathematics and Computer Science (EEMCS)
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