Detachment and sonoporation of adherent HeLa-cells by shock wave-induced cavitation


Ohl, Claus-Dieter and Wolfrum, Bernhard (2003) Detachment and sonoporation of adherent HeLa-cells by shock wave-induced cavitation. Biochimica et Biophysica Acta (BBA) - General Subjects, 1624 (1-3). pp. 131-138. ISSN 0304-4165

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Abstract:The interaction of lithotripter-generated shock waves with adherent cells is investigated using high-speed optical techniques. We show that shock waves permeabilize adherent cells in vitro through the action of cavitation bubbles. The bubbles are formed in the trailing tensile pulse of a lithotripter-generated shock wave where the pressure drops below the vapor pressure. Upon collapse of cavitation bubbles, a strong flow field is generated which accounts for two effects: first, detachment of cells from the substrate; and second, the temporary opening of cell membranes followed by molecular uptake, a process called sonoporation. Comparison of observed cell detachment with results from a theoretical model considering peeling cell detachment by a wall jet-induced shear stress shows reasonable agreement.
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Copyright:© 2003 Elsevier
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Metis ID: 216922