State of the art of shear driven chromatography: Advantages and limitations


Fekete, Veronika and Clicq, David and De Malsche, Wim and Gardeniers, Han and Desmet, Gert (2007) State of the art of shear driven chromatography: Advantages and limitations. Journal of Chromatography A, 1149 (1). pp. 2-11. ISSN 0021-9673

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Abstract:The present paper reports on the experimental difficulties encountered when trying to realize the full potential of shear-driven chromatography in nanochannels. While it theoretically offers the possibility to yield over 10,000 plates per centimetre in a few seconds, the practical realization of this potential requires a detector miniaturisation that is carried to the extreme combined with very high sampling rates. In the present study, a charge coupled device camera and a photomultiplier tube combined with pinhole were tested as detector. Despite the fact that the photomultiplier tube could offer a higher sampling rate and a better sensitivity, the charge coupled device turned out to be better suited for the current set-up because of inevitable problems with the stray-light transported through the glass channel wall. The chemistry of the separation surface was additionally studied getting more homogenous coating, thus higher separation efficiency. Having also carried out a number of mechanical improvements, it is now possible to measure separations at a distance of 8 mm downstream from the injection point. This is four times further downstream than ever before while realizing a four components mixture separation in less than 1.5 s, with a plate generation velocity of about 2000–7000 plates per second depending on the sample.

Item Type:Article
Additional information:26th International Symposium on Chromatography
Copyright:© 2007 Elsevier B.V
Science and Technology (TNW)
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