Directed Formation of Micro- and Nanoscale Patterns of Functional Light-Harvesting LH2 Complexes

Reynolds, Nicholas P. and Janusz, Stefan and Escalante-Marun, Maryana and Timney, John and Ducker, Robert E. and Olsen, John D. and Otto, Cees and Subramaniam, Vinod and Leggett, Graham J. and Hunter, C. Neil (2007) Directed Formation of Micro- and Nanoscale Patterns of Functional Light-Harvesting LH2 Complexes. Journal of the American Chemical Society, 129 (47). pp. 14625-14631. ISSN 0002-7863

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Abstract:	The precision placement of the desired protein components on a suitable substrate is an essential prelude to any hybrid “biochip” device, but a second and equally important condition must also be met: the retention of full biological activity. Here we demonstrate the selective binding of an optically active membrane protein, the light-harvesting LH2 complex from Rhodobacter sphaeroides, to patterned self-assembled monolayers at the micron scale and the fabrication of nanometer-scale patterns of these molecules using near-field photolithographic methods. In contrast to plasma proteins, which are reversibly adsorbed on many surfaces, the LH2 complex is readily patterned simply by spatial control of surface polarity. Near-field photolithography has yielded rows of light-harvesting complexes only 98 nm wide. Retention of the native optical properties of patterned LH2 molecules was demonstrated using in situ fluorescence emission spectroscopy.
Item Type:	Article
Copyright:	© 2007 American Chemical Society
Faculty:	Science and Technology (TNW)
Research Group:	Medical Cell BioPhysics (MCBP) Nano-Biophysics (NBP)
Link to this item:	http://purl.utwente.nl/publications/74644
Official URL:	http://dx.doi.org/10.1021/ja073658m
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