Viability of dielectrophoretically trapped neural cortical cells in culture


Heida, T. and Vulto, P. and Rutten, W.L.C. and Marani, E. (2001) Viability of dielectrophoretically trapped neural cortical cells in culture. Journal of Neuroscience Methods, 110 (1-2). pp. 37-44. ISSN 0165-0270

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Abstract:Negative dielectrophoretic trapping of neural cells is an efficient way to position neural cells on the electrode sites of planar micro-electrode arrays. The preservation of viability of the neural cells is essential for this approach. This study investigates the viability of postnatal cortical rat cells that were dielectrophoretically trapped. Morphological characteristics as well as the ratio of the number of outgrowing to the number of non-outgrowing cortical cells were used to compare the viability of trapped cells to that of non-exposed cells. The morphological characteristics include the area of the cell, representing adhesive properties, and the number and length of the processes, as a measure for functional recovery. The results presented in this paper show that the viable state of dielectrophoretically trapped postnatal cortical rat cells under the conditions used was similar to that of non-exposed cells.
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Copyright:© 2001 Elsevier
Electrical Engineering, Mathematics and Computer Science (EEMCS)
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