In vitro and in vivo evaluation of gelatin–chondroitin sulphate hydrogels for controlled release of antibacterial proteins


Kuijpers, A.J. and Wachem, P.B. van and Luyn, M.J.A. van and Brouwer, L.A. and Engbers, G.H.M. and Krijgsveld, J. and Zaat, S.A.J. and Dankert, J. and Feijen, J. (2000) In vitro and in vivo evaluation of gelatin–chondroitin sulphate hydrogels for controlled release of antibacterial proteins. Biomaterials, 21 (17). pp. 1763-1772. ISSN 0142-9612

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Abstract:Chemically cross-linked gelatin–chondroitin sulphate (ChS) hydrogels, impregnated in Dacron, were evaluated as drug delivery systems for antibacterial proteins. The gelatin–chondroitin sulphate gels, plain or impregnated in Dacron, were cross-linked with a water-soluble carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The release of lysozyme and recombinant thrombocidin (rTC-1), an antibacterial protein derived from human blood platelets, from the gelatin–ChS gels in Dacron in phosphate-buffered saline at 37°C was determined, and compared to the release from gelatin gels in Dacron and plain gelatin–ChS gels. The incorporation of chondroitin sulphate into gelatin gels, caused a marked increase in lysozyme loading capacity, and a slower release rate. The relative release profiles for rTC-1 and lysozyme were equal for cross-linked gelatin as well as for cross-linked gelatin–ChS gels. Furthermore, rTC-1 showed no loss of antibacterial activity after 1 week of release. The lysozyme concentration profiles in the samples and in the surrounding medium as a function of time were calculated using mathematical solutions for Ficks second law of diffusion for a semi-infinite composite medium, which is a schematic representation of a slab in a surrounding medium. The biocompatibility and degradation of the Dacron matrices impregnated with gelatin–ChS gels was studied after implantation in subcutaneous pockets in rats. Chemically cross-linked gelatin–ChS gels showed a mild tissue reaction, and almost complete degradation within 18 weeks of implantation.
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Copyright:© 2000 Elsevier
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