Adsorption of proteins from plasma at polyester non-wovens

Klomp, A.J.A. and Engbers, G.H.M. and Mol, J. and Terlingen, J.G.A. and Feijen, J. (1999) Adsorption of proteins from plasma at polyester non-wovens. Biomaterials, 20 (13). pp. 1203-1211. ISSN 0142-9612

PDF
Restricted to UT campus only: Request a copy
312Kb

Abstract:	Polyester non-wovens in filters for the removal of leukocytes from platelet concentrates (PCs) must be platelet compatible. In PC filtration, the adsorption of proteins at the plasma–non-woven interface can be of great importance with respect to the yield of platelets. Unmodified and radio frequency glow discharge (RFGD) treated poly(ethylene terephthalate) non-woven (NW-PET) and two commercial surface-modified non-wovens were contacted with human plasma. Protein desorption by sodium dodecyl sulphate (SDS) was evaluated by X-ray photoelectron spectroscopy (XPS). The desorbed proteins were characterized by gel electrophoresis and immunoblotting. Compared to the commercial surface-modified non-wovens, unmodified and RFGD-treated NW-PETs adsorbed a relatively high amount of protein. Significantly more protein was removed from the hydrophobic NW-PET by SDS than from the hydrophilic RFGD-treated non-wovens. RFGD treatment of NW-PET reduces the reversibility of protein adsorption. Less albumin and fibrinogen were removed from the RFGD-treated non-wovens than from NW-PET. In addition, a large amount of histidine-rich glycoprotein was removed from RFGD-treated non-wovens, but not from NW-PET. The different behaviour of RFGFD-treated non-wovens towards protein adsorption is probably caused by differences in the chemical reactivity of the non-woven surfaces.
Item Type:	Article
Copyright:	© 1999 Elsevier
Faculty:	Science and Technology (TNW)
Research Group:	Polymer Chemistry and BioMaterials (PBM)
Link to this item:	http://purl.utwente.nl/publications/74032
Official URL:	http://dx.doi.org/10.1016/S0142-9612(99)00009-5
Export this item as:	BibTeX EndNote HTML Citation Reference Manager

Show download statistics for this publication

Repository Staff Only: item control page

Metis ID: 105674