Flow cytometric analysis of the uptake of low-density lipoprotein by endothelial cells in microfluidic channels

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Meer, A.D. van der and Vermeul, K. and Poot, A.A. and Feijen, J. and Vermes, I. (2010) Flow cytometric analysis of the uptake of low-density lipoprotein by endothelial cells in microfluidic channels. Cytometry Part A, 77 (10). pp. 971-975. ISSN 0196-4763

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Abstract:Acceptance of microfluidic technology in everyday laboratory practice by biologists is still low. One of the reasons for this is that the technology combines poorly with standard cell biological and biochemical analysis tools. Flow cytometry is an example of a conventional analytical tool that is considered to be incompatible with microfluidic technology and its inherent small sample sizes. In this study, it is shown that properly designed microfluidic devices contain cell populations that are large enough to be analyzed by flow cytometry. To illustrate this, the uptake of fluorescent human low-density lipoprotein (LDL) by human endothelial cells that were cultured in a microfluidic channel was analyzed. It was found that the uptake of LDL by the cells increased linearly over time. Moreover, the uptake decreased when cells were pretreated with fluid shear stress inside the microfluidic devices. This study shows that microfluidic technology can be combined with conventional flow cytometry, while retaining the advantages of working with microfluidics such as low reagent use and dynamic cell culture conditions. This approach of combining microfluidic technology with conventional laboratory tools may contribute to greater acceptance of microfluidic devices in biological research.
Item Type:Article
Copyright:© 2010 International Society for Advancement of Cytometry
Faculty:
Electrical Engineering, Mathematics and Computer Science (EEMCS)
Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/73994
Official URL:http://dx.doi.org/10.1002/cyto.a.20937
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