Preparation and characterization of microspheres of albumin-heparin conjugates

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Kwon, Glen S. and Bae, You Han and Kim, Sung Wan and Cremers, Harry and Feijen, Jan (1991) Preparation and characterization of microspheres of albumin-heparin conjugates. Journal of Colloid and Interface Science, 143 (2). pp. 501-512. ISSN 0021-9797

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Abstract:Albumin-heparin microspheres have been prepared as a new drug carrier. A soluble albumin-heparin conjugate was synthesized by forming amide bonds between human serum albumin and heparin. After purification the albumin-heparin conjugate was crosslinked in a water-in-oil emulsion to form albumin-heparin microspheres. The composition of the conjugate was determined by amino acid analysis. The swelling properties of albumin-heparin microspheres were investigated as a function of pH and ionic strength and compared with albumin microspheres. Albumin-heparin and albumin microspheres exhibited stimuli-sensitive swelling. Both microsphere systems exhibited low swelling at low pH and high swelling at higher pH caused by ionization of amino acids of serum albumin. The swelling of albumin-heparin microspheres was more sensitive toward ionic strength than that of albumin microspheres. This was due to the greater negative charge of the albumin-heparin microspheres. Surfaces of albumin-heparin and albumin microspheres were characterized by ESCA, contact angle measurements, electrophoresis, and scanning electron microscopy. Surface analysis indicated the presence of heparin at the albumin-heparin microsphere/water interface.
Item Type:Article
Copyright:© 1991 Elsevier
Faculty:
Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/73004
Official URL:http://dx.doi.org/10.1016/0021-9797(91)90283-E
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Metis ID: 105238