Biomimetic calcium phosphate coatings on recombinant spider silk fibres

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Yang, Liang and Hedhammar, My and Blom, Tobias and Leifer, Klaus and Johansson, Jan and Habibovic, Pamela and Blitterswijk van, Clemens A. (2010) Biomimetic calcium phosphate coatings on recombinant spider silk fibres. Biomedical Materials, 5 (4). 045002. ISSN 1748-6041

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Abstract:Calcium phosphate ceramic coatings, applied on surfaces of metallic and polymeric biomaterials, can improve their performance in bone repair and regeneration. Spider silk is biocompatible, strong and elastic, and hence an attractive biomaterial for applications in connective tissue repair. Recently, artificial spider silk, with mechanical and structural characteristics similar to those of native spider silk, has been produced from recombinant minispidroins. In the present study, supersaturated simulated body fluid was used to deposit calcium phosphate coatings on recombinant spider silk fibres. The mineralization process was followed in time using scanning electron microscopy equipped with an energy dispersive x-ray (EDX) detector and Raman spectroscope. Focused ion beam technology was used to produce a cross section of a coated fibre, which was further analysed by EDX. Preliminary in vitro experiments using a culture of bone marrow-derived human mesenchymal stem cells (hMSCs) on coated fibres were also performed. This study showed that recombinant spider silk fibres were successfully coated with a homogeneous and thick crystalline calcium phosphate layer. In the course of the mineralization process from modified simulated body fluid, sodium chloride crystals were first deposited on the silk surface, followed by the deposition of a calcium phosphate layer. The coated silk fibres supported the attachment and growth of hMSCs.
Item Type:Article
Copyright:© IOP Publishing 2010
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Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/72976
Official URL:http://dx.doi.org/10.1088/1748-6041/5/4/045002
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