Dual-point dual-wavelength fluorescence monitoring of DNA separation in a lab on a chip


Dongre, Chaitanya and Weerd, Jasper van and Bellini, Nicola and Osellame, Roberto and Cerullo, Giulio and Weeghel, Rob van and Hoekstra, Hugo J.W.M. and Pollnau, Markus (2010) Dual-point dual-wavelength fluorescence monitoring of DNA separation in a lab on a chip. Biomedical Optics Express, 1 (2). pp. 729-735. ISSN 2156-7085

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Abstract:We present a simple approach in electrophoretic DNA separation and fluorescent monitoring that allows to identify the insertion or deletion of base-pairs in DNA probe molecules from genetic samples, and to perform intrinsic calibration/referencing for highly accurate DNA analysis. The principle is based on dual-point, dual-wavelength laser-induced fluorescence excitation using one or two excitation windows at the intersection of integrated waveguides and microfluidic channels in an optofluidic chip and a single, color-blind photodetector, resulting in a limit of detection of ~200 pM for single-end-labeled DNA molecules. The approach using a single excitation window is demonstrated experimentally, while the option exploiting two excitation windows is proposed theoretically.
Item Type:Article
Additional information:Open access article
Copyright:© 2010 Optical Society of America
Electrical Engineering, Mathematics and Computer Science (EEMCS)
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Link to this item:http://purl.utwente.nl/publications/72873
Official URL:https://doi.org/10.1364/BOE.1.000729
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