Preparation of Biomolecule Microstructures and Microarrays by Thiol–ene Photoimmobilization

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Weinrich, Dirk and Köhn, Maja and Jonkheijm, Pascal and Westerlind, Ulrika and Dehmelt, Leif and Engelkamp, Hans and Christianen, Peter C.M. and Kuhlmann, Jürgen and Maan, Jan C. and Nüsse, Dirk and Schröder, Hendrik and Wacker, Ron and Voges , Edgar and Breinbauer, Rolf and Kunz, Horst and Niemeyer, Christof M. and Waldmann, Herbert (2010) Preparation of Biomolecule Microstructures and Microarrays by Thiol–ene Photoimmobilization. ChemBioChem, 11 (2). pp. 235-247. ISSN 1439-4227

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Abstract:A mild, fast and flexible method for photoimmobilization of biomolecules based on the light-initiated thiol–ene reaction has been developed. After investigation and optimization of various surface materials, surface chemistries and reaction parameters, microstructures and microarrays of biotin, oligonucleotides, peptides, and MUC1 tandem repeat glycopeptides were prepared with this photoimmobilization method. Furthermore, MUC1 tandem repeat glycopeptide microarrays were successfully used to probe antibodies in mouse serum obtained from vaccinated mice. Dimensions of biomolecule microstructures were shown to be freely controllable through photolithographic techniques, and features down to 5 μm in size covering an area of up to 75×25 mm were created. Use of a confocal laser microscope with a UV laser as UV-light source enabled further reduction of biotin feature size opening access to nanostructured biochips.
Item Type:Article
Copyright:© 2010 Wiley
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Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/72760
Official URL:http://dx.doi.org/10.1002/cbic.200900559
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