Competitive immunological detection of progesterone by means of the ion-step induced response of an immunoFET

Schasfoort, R.B.M. and Keldermans, C.E.J.M. and Kooyman, R.P.H. and Bergveld, P. and Greve, J. (1990) Competitive immunological detection of progesterone by means of the ion-step induced response of an immunoFET. Sensors and Actuators B: Chemical, 1 (1-6). pp. 368-372. ISSN 0925-4005

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Abstract:	The ion-step method was originally designed to measure immunoreactions via the change in charge density which occurs in an antibody loaded membrane, deposited on an ISFET, upon reaction with a charged antigen. In this paper we show the possibility of detecting an immunoreaction involving a non-charged antigen, progesterone, by using competitive binding of progesterone and a charged progesterone-lysozyme conjugate in the membrane. Both the immunoreaction and the non-specific binding could unequivocally be measured. The detection limit was approximately 10−8 M of progesterone in the sample solution. This proves that the ion-step method is suitable for detection of both charged and non-charged antigens.
Item Type:	Article
Copyright:	© 1990 Elsevier
Faculty:	Science and Technology (TNW)
Research Group:	Medical Cell BioPhysics (MCBP) Nano-Biophysics (NBP)
Link to this item:	http://purl.utwente.nl/publications/72673
Official URL:	http://dx.doi.org/10.1016/0925-4005(90)80232-O
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