Competitive immunological detection of progesterone by means of the ion-step induced response of an immunoFET

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Schasfoort, R.B.M. and Keldermans, C.E.J.M. and Kooyman, R.P.H. and Bergveld, P. and Greve, J. (1990) Competitive immunological detection of progesterone by means of the ion-step induced response of an immunoFET. Sensors and Actuators B: Chemical, 1 (1-6). pp. 368-372. ISSN 0925-4005

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Abstract:The ion-step method was originally designed to measure immunoreactions via the change in charge density which occurs in an antibody loaded membrane, deposited on an ISFET, upon reaction with a charged antigen. In this paper we show the possibility of detecting an immunoreaction involving a non-charged antigen, progesterone, by using competitive binding of progesterone and a charged progesterone-lysozyme conjugate in the membrane. Both the immunoreaction and the non-specific binding could unequivocally be measured. The detection limit was approximately 10−8 M of progesterone in the sample solution. This proves that the ion-step method is suitable for detection of both charged and non-charged antigens.
Item Type:Article
Copyright:© 1990 Elsevier
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Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/72673
Official URL:http://dx.doi.org/10.1016/0925-4005(90)80232-O
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