Background free CARS imaging by local phase detection


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Jurna, M. and Korterik, J.P. and Otto, C. and Herek, L. and Offerhaus, H.L. (2009) Background free CARS imaging by local phase detection. In: Multiphoton microscopy in the biomedical sciences IX. Proceedings of SPIE, 7183 . SPIE, p. 718310. ISBN 9780819474292

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Abstract:In this article we show that heterodyne CARS, based on a controlled and stable phase-preserving chain, can be used to measure amplitude and phase information of molecular vibration modes. The technique is validated by a comparison of the imaginary part of the heterodyne CARS spectrum to the spontaneous Raman spectrum of polyethylene. The detection of the phase allows for rejection of the non-resonant background from the data. The resulting improvement of the signal to noise ratio is shown by measurements on a sample containing lipid.
Item Type:Book Section
Copyright:©2009 COPYRIGHT SPIE--The International Society for Optical Engineering.
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Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/72659
Official URL:http://dx.doi.org/10.1117/12.807714
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Metis ID: 255565