Background free CARS imaging by local phase detection


Jurna, M. and Korterik, J.P. and Otto, C. and Herek, L. and Offerhaus, H.L. (2009) Background free CARS imaging by local phase detection. In: Ammasi Periasamy & Peter T.C. So (Eds.), Multiphoton microscopy in the biomedical sciences IX. Proceedings of SPIE, 7183 . SPIE, p. 718310. ISBN 9780819474292

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Abstract:In this article we show that heterodyne CARS, based on a controlled and stable phase-preserving chain, can be used to measure amplitude and phase information of molecular vibration modes. The technique is validated by a comparison of the imaginary part of the heterodyne CARS spectrum to the spontaneous Raman spectrum of polyethylene. The detection of the phase allows for rejection of the non-resonant background from the data. The resulting improvement of the signal to noise ratio is shown by measurements on a sample containing lipid.
Item Type:Book Section
Copyright:©2009 COPYRIGHT SPIE--The International Society for Optical Engineering.
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