Process Design for Enzymatic Adipyl-7-ADCA Hydrolysis


Schroën, Catharina Gerarda Petronella Henrica and Nierstrasz, Vincent Adriaan and Bosma, Rouke and Dijkstra, Zwaantje Johanna and VandeSandt, Emilius Johannes Albertus Xavierius and Beeftink, Hendrik Harmen and Tramper, Johannes (2002) Process Design for Enzymatic Adipyl-7-ADCA Hydrolysis. Biotechnology Progress, 18 (4). pp. 745-751. ISSN 8756-7938

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Abstract:Adipyl-7-ADCA is a new source for 7-aminodeacetoxycephalosporanic acid (7-ADCA), one of the substrates for antibiotics synthesis. In this paper, a novel process for enzymatic 7-ADCA production is presented. The process consists of a reactor, a crystallization step, a membrane separation step, and various recycle loops. The reactor can either be operated batch-wise or continuously; with both types of processing high yields can be obtained. For batch reactors chemical degradation of 7-ADCA can be neglected. For continuous reactors, chemical stability of 7-ADCA is a factor to be taken into account. However, it was shown that the reaction conditions and reactor configuration could be chosen in such a way that also for continuous operation chemical degradation is not important. Downstream processing consisted of crystallization of 7-ADCA at low pH, followed by a nanofiltration step with which, at low pH, adipic acid could be separated from adipyl-7-ADCA and 7-ADCA. The separation mechanism of the nanofilter is based on size exclusion combined with charge effects. Application of this filtration step opens possibilities for recycling components to various stages of the process. Adipic acid can be recycled to the fermentation stage of the process while both adipyl-7-ADCA and 7-ADCA can be returned to the hydrolysis reactor. In this way, losses of substrates and product can be minimized.
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Copyright:© 2008 Wiley
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