High-resolution electrophoretic separation and integrated-waveguide excitation of fluorescent DNA molecules in a lab on a chip

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Dongre, Chaitanya and Weerd, Jasper van and Besselink, Geert A.J. and Weeghel, Rob van and Martinez-Vazquez, Rebecca and Osellame, Roberto and Cerullo, Giulio and Cretich, Marina and Chiari, Marcella and Hoekstra, Hugo J.W.M. and Pollnau, Markus (2010) High-resolution electrophoretic separation and integrated-waveguide excitation of fluorescent DNA molecules in a lab on a chip. Electrophoresis, 31 (15). pp. 2584-2588. ISSN 0173-0835

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Abstract:By applying integrated-waveguide laser excitation to an optofluidic chip, fluorescently labeled DNA molecules of 12 or 17 different sizes are separated by CE with high operating speed and low sample consumption of ~600 pL. When detecting the fluorescence signals of migrating DNA molecules with a PMT, the LOD is as low as 2.1 pM. In the diagnostically relevant size range (~150–1000 base-pairs) the molecules are separated with reproducibly high sizing accuracy (>99%) and the plug broadening follows Poissonian statistics. Variation of the power dependence of migration time on base-pair size – probably with temperature and condition of the sieving gel matrix – indicates that the capillary migration cannot be described by a simple physical law. Integrated-waveguide excitation of a 12-µm narrow microfluidic segment provides a spatio-temporal resolution that would, in principle, allow for a 20-fold better accuracy than the currently supported by state-of-the-art electrophoretic separation in microchips, thereby demonstrating the potential of this integrated optical approach to fulfill the resolution demands of future electrophoretic microchips.
Item Type:Article
Copyright:© 2010 WILEY-VCH Verlag
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Electrical Engineering, Mathematics and Computer Science (EEMCS)
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Link to this item:http://purl.utwente.nl/publications/72446
Official URL:http://dx.doi.org/10.1002/elps.201000126
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