Electrokinetic label-free screening chip: a marriage of multiplexing and high throughput analysis using surface plasmon resonance imaging

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Krishnamoorthy, Ganeshram and Carlen, Edwin T. and Bomer, Johan G. and Wijnperle, Daniël and Boer de, Hans L. and Berg van den, Albert and Schasfoort, Richard B.M. (2010) Electrokinetic label-free screening chip: a marriage of multiplexing and high throughput analysis using surface plasmon resonance imaging. Lab on a Chip, 10 (8). pp. 986-990. ISSN 1473-0197

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Abstract:We present an electrokinetic label-free biomolecular screening chip (Glass/PDMS) to screen up to 10 samples simultaneously using surface plasmon resonance imaging (iSPR). This approach reduces the duration of an experiment when compared to conventional experimental methods. This new device offers a high degree of parallelization not only for analyte samples, but also for multiplex analyte interactions where up to 90 ligands are immobilized on the sensing surface. The proof of concept has been demonstrated with well-known biomolecular interactant pairs. The new chip can be used for high throughput screening applications and kinetics parameter extraction, simultaneously, of interactant-protein complex formation
Item Type:Article
Copyright:© 2010 RSC Publishing
Faculty:
Electrical Engineering, Mathematics and Computer Science (EEMCS)
Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/72394
Official URL:http://dx.doi.org/10.1039/c000705f
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