Electrokinetic Lab-on-a-BioChip for Multi-ligand/Multi-analyte Biosensing

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Krishnamoorthy, Ganeshram and Carlen, Edwin T. and Boer, Hans L. de and Berg, Albert van den and Schasfoort, Richard B.M. (2010) Electrokinetic Lab-on-a-BioChip for Multi-ligand/Multi-analyte Biosensing. Analytical Chemistry, 82 (10). pp. 4145-4150. ISSN 0003-2700

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Abstract:We present a simple electrokinetic lab-on-a-biochip (EKLB) with four microchannels integrated with a surface plasmon resonance imaging (iSPR) label-free biosensor that is operated using a single electrical voltage for the simultaneous transport of reagents in all microchannels without conventional fluidic plumbing. We demonstrate the utility of the simple approach with various biosensing experiments, including single injection kinetics (multiple varied ligand densities and single analyte concentration), one shot kinetics (single ligand densities and multiple varied analyte concentrations), and multi-ligand/multi-analyte detection. In all cases, the binding kinetics and affinity were extracted using a conventional 1:1 interaction model. Since the reagent transport is done with a single electrical voltage source, scaling up to hundreds to thousands of simultaneous experiments is straightforward.
Item Type:Article
Copyright:© 2010 American Chemical Society
Faculty:
Electrical Engineering, Mathematics and Computer Science (EEMCS)
Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/72393
Official URL:http://dx.doi.org/10.1021/ac1003163
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Metis ID: 269017