Quantitative analysis of thiols in consumer products on a microfluidic CE chip with fluorescence detection

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Revermann, Tobias and Götz, Sebastian and Karst, Uwe (2007) Quantitative analysis of thiols in consumer products on a microfluidic CE chip with fluorescence detection. Electrophoresis, 28 (7). pp. 1154-1160. ISSN 0173-0835

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Abstract:A microchip CE-based method for the quantification of the thiols mercaptoethanoic acid (MAA) and 2-mercaptopropionic acid (2-MPA) in depilatory cream and cold wave lotions was developed. The thiols were first derivatized with the fluorogenic reagent ammonium-7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD-F). The derivatives were separated within only 20 s by microchip CE and detected by their fluorescence. Conventional CE with diode array detection and LC with fluorescence detection were used for validation. The internal standard 3-mercaptopropionic acid (3-MPA) provided RSDs of multiple injections of only 4% or less for the MCE approach. LOD is 2 µM, LOQ 6 µM, and the linear range comprises nearly three decades of concentration starting at the LOQ.
Item Type:Article
Copyright:© 2007 Wiley InterScience
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Link to this item:http://purl.utwente.nl/publications/72255
Official URL:http://dx.doi.org/10.1002/elps.200600419
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