Creating Nanopatterns of His-Tagged Proteins on Surfaces by Nanoimprint Lithography Using Specific NiNTA-Histidine Interactions

Maury, Pascale and Escalante, Maryana and Peter, Mária and Reinhoudt, David N. and Subramaniam, Vinod and Huskens, Jurriaan (2007) Creating Nanopatterns of His-Tagged Proteins on Surfaces by Nanoimprint Lithography Using Specific NiNTA-Histidine Interactions. Small, 3 (9). pp. 1584-1592. ISSN 1613-6829

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Abstract:	Directed assembly of the DsRed FT protein is demonstrated on self-assembled monolayers (SAMs) on silicon substrates patterned by nanoimprint lithography. Initially, the DsRed protein is attached using electrostatic interactions on both topographical (polymer) templates with an amino functionalization and on chemically patterned (flat) substrates. In a second experiment, a patterned NiNTA SAM is used in order to attach the DsRed FT protein via supramolecular interactions, taking advantage of the histidine functionalization of the DsRed FT protein. The NTA SAM is formed on silicon oxide using a multistep covalent process. Patterning of the NTA SAM is performed using nanoimprint lithography. The DsRed FT protein is attached on the patterned NTA layer after treating this with a NiII solution. Moreover, the histidine-NiNTA binding may be reversed by removing the Ni using EDTA or by competition using imidazole. The regeneration and reuse of the substrate by subsequently attaching and removing two different histidine-functionalized proteins from the patterned NTA is shown by fluorescence microscopy.
Item Type:	Article
Copyright:	© 2007 Wiley InterScience
Faculty:	Science and Technology (TNW)
Research Group:	Nano-Biophysics (NBP) Molecular Nanofabrication (MNF)
Link to this item:	http://purl.utwente.nl/publications/72202
Official URL:	http://dx.doi.org/10.1002/smll.200700046
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