Micromechanical Testing of Individual Collagen Fibrils

Rijt van der, Joost A.J. and Werf van der, Kees O. and Bennink, Martin L. and Dijkstra, Pieter J. and Feijen, Jan (2005) Micromechanical Testing of Individual Collagen Fibrils. Macromolecular Bioscience, 6 (9). pp. 697-702. ISSN 1616-5187

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Abstract:	A novel method based on AFM was used to attach individual collagen fibrils between a glass surface and the AFM tip, to allow force spectroscopy studies of these. The fibrils were deposited on glass substrates that are partly coated with Teflon AF®. A modified AFM tip was used to accurately deposit epoxy glue droplets on either end of the collagen fibril that cross the glass-Teflon AF® interface, as to such attach it with one end to the glass and the other end to the AFM tip. Single collagen fibrils have been mechanically tested in ambient conditions and were found to behave reversibly up to stresses of 90 MPa. Within this regime a Young's modulus of 2-7 GPa was obtained. In aqueous media, the collagen fibrils could be tested reversibly up to about 15 MPa, revealing Young's moduli ranging from 0.2 to at most 0.8 GPa.
Item Type:	Article
Copyright:	© 2005 Wiley InterScience
Faculty:	Science and Technology (TNW)
Research Group:	Nano-Biophysics (NBP) Polymer Chemistry and BioMaterials (PBM)
Link to this item:	http://purl.utwente.nl/publications/72053
Official URL:	http://dx.doi.org/10.1002/mabi.200600063
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