Synthetic scaffold morphology controls human dermal connective tissue formation

Share/Save/Bookmark

Wang, Hongjun and Pieper, Jeroen and Peters, Fabienne and Blitterswijk van, Clemens A. and Lamme, Evert N. (2005) Synthetic scaffold morphology controls human dermal connective tissue formation. Journal of Biomedical Materials Research. Part A, 74 (4). pp. 523-532. ISSN 1549-3296

[img]PDF
Restricted to UT campus only
: Request a copy
728Kb
Abstract:Engineering tissues in bioreactors is often hampered by disproportionate tissue formation at the surface of scaffolds. This hinders nutrient flow and retards cell proliferation and tissue formation inside the scaffold. The objective of this study was to optimize scaffold morphology to prevent this from happening and to determine the optimal scaffold geometric values for connective tissue engineering. After comparing lyophilized crosslinked collagen, compression molded/salt leached PEGT/PBT copolymer and collagen-PEGT/PBT hybrid scaffolds, the PEGT/PBT scaffold was selected for optimization. Geometric parameters were determined using SEM, microcomputed tomography, and flow permeability measurements. Fibroblast were seeded and cultured under dynamic flow conditions for 2 weeks. Cell numbers were determined using CyQuant DNA assay, and tissue distribution was visualized in H&E- and Sirius Red-stained sections. Scaffolds 0.5 and 1.5 mm thick showed bridged connected tissue from top-to-bottom, whereas 4-mm-thick scaffolds only revealed tissue ingrowth until a maximum depth of 0.6-0.8 mm. Rapid prototyped scaffold were used to assess the maximal void space (pore size) that still could be filled with tissue. Tissue bridging between fibers was only found at fiber distances 401 ± 60 m, whereas filling of void spaces in 3D-deposited scaffolds only occurred at distances ≤273 ± 55 µm. PEGT/PBT scaffolds having similar optimal porosities, but different average interconnected pore sizes of 142 ± 50, 160 ± 56 to 191 ± 69 µm showed comparable seeding efficiencies at day 1, but after 2 weeks the total cell numbers were significantly higher in the scaffolds with intermediate and high interconnectivity. However, only scaffolds with an intermediate interconnectivity revealed homogenous tissue formation throughout the scaffold with complete filling of all pores. In conclusion, significant amount of connective tissue was formed within 14 days using a dynamic culture process that filled all void spaces of a PEGT/PBT scaffolds with the following geometric parameters: thickness 1.5-1.6 mm, pore size range 90-360 µm, and average interconnecting pore size of 160 ± 56 µm.
Item Type:Article
Copyright:© 2005 Wiley InterScience
Faculty:
Science and Technology (TNW)
Research Group:
Link to this item:http://purl.utwente.nl/publications/72035
Official URL:http://dx.doi.org/10.1002/jbm.a.30232
Export this item as:BibTeX
EndNote
HTML Citation
Reference Manager

 

Repository Staff Only: item control page

Metis ID: 230764