Remineralization of demineralized albumin-calcium phosphate coatings


Liu, Y. and Hunziker, E.B. and Layrolle, P. and Blitterswijk, C.A. van and Calvert, P.D. and Groot, K. de (2003) Remineralization of demineralized albumin-calcium phosphate coatings. Journal of Biomedical Materials Research. Part A, 67 (4). pp. 1155-1162. ISSN 1549-3296

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Abstract:Calcium phosphate and bovine serum albumin were coprecipitated (under physiological conditions of temperature and pH) upon the surfaces of titanium-alloy samples, which thereby became coated with a dense, proteinaceous mineral layer 30-50 µm in thickness. Dissolution of the inorganic phase by treatment with acidic saline yielded a self-supporting protein scaffold, 7-10 m in thickness. Energy-dispersive X-ray analysis and Fourier-transform infrared spectroscopy confirmed the absence of inorganic components from the demineralized albumin scaffolds. When titanium-alloy samples bearing these demineralized protein scaffolds were immersed in a supersaturated solution of calcium phosphate (again at physiological temperature and pH), they remineralized. These redux albumin-calcium phosphate layers corresponded in thickness to the original coatings. When titanium-alloy discs bearing the demineralized protein scaffolds were implanted ectopically (subcutaneously) in mice, they, too, remineralized. No uniform mineral layer was deposited upon the surfaces of naked titanium-alloy implants. To the best of our knowledge, this is the first demonstration of remineralization within the interstices of a noncollagenous protein scaffold, either in vitro or in vivo.
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Copyright:© 2003 Wiley InterScience
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Metis ID: 217263