CARS and Raman spectroscopy of function-related conformational changes of chymotrypsin

Brandt, N.N. and Chikishev, A.Yu. and Greve, J. and Koroteev, N.I. and Otto, C. and Sakodynskaya, I.K. (2000) CARS and Raman spectroscopy of function-related conformational changes of chymotrypsin. Journal of Raman Spectroscopy, 31 (8-9). pp. 731-737. ISSN 0377-0486

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Abstract:	We report on the comparative analysis of the conformation-sensitive bands of free enzyme (chymotrypsin), liganded enzyme (chymotrypsin anthranilate) and enzyme complex with 18-crown-6. The studies were carried out by Raman scattering spectroscopy and polarization-sensitive coherent anti-Stokes Raman scattering spectroscopy (CARS). The bands of tyrosine and tryptophan residues, disulfide bridges and amide I and amide III were analyzed. It is concluded that the conformation of the enzyme changes much more in the case of liganding than in the case of the formation of a complex with the crown ether.
Item Type:	Article
Copyright:	© 2000 Wiley InterScience
Faculty:	Science and Technology (TNW)
Research Group:	Medical Cell BioPhysics (MCBP)
Link to this item:	http://purl.utwente.nl/publications/71574
Official URL:	http://dx.doi.org/10.1002/1097-4555(200008/09)31:8/9<731::AID-JRS609>3.0.CO;2-0
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